-acetyl spinoff. This result is in step with the formation or collapse with the tetrahedral intermediate as the speed limiting stage within the catalytic system of NagA.
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For The only histidine substitutions, the N61H mutant is comparable in catalytic exercise to the double mutant Whilst the Q59H mutant is diminished by below an order of magnitude relative on the wild type enzyme. With the substitutions at Asn-61, the alternative with the alanine is significantly considerably less disruptive than the adjust into a histidine.
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